Protein analysis. (A) Typical flowchart for protein analysis after

September 21,2024

Download scientific diagram | | Protein analysis. (A) Typical flowchart for protein analysis after small-scale expression test. Parameters to be considered are highlighted in blue. (B) Small-scale expression test of the nonstructural protein 2 (NS2) from hepatitis C virus (HCV). This membrane protein was produced in the absence or presence of various detergents at a 0.1% concentration (w/v). Samples were analyzed by SDS-PAGE followed by Coomassie blue staining (upper panels) and Western blotting with an antibody against the Strep-tag II fused at the C-terminus of NS2 (lower panels). CFS, total cell-free sample; pellet, pellet obtained after centrifugation of CFS; SN-beads, supernatant obtained after centrifugation of CFS and incubated with Strep-Tactin magnetic beads to capture Strep-tag II-tagged NS2 protein; −, negative control (no NS2); +, positive control (NS2 expressed in the absence of detergent). The black arrowheads indicate NS2, adapted from Fogeron et al. (2015a). (C) SDS-PAGE analysis followed by Coomassie blue staining of the different steps from the affinity purification of the NS2 membrane protein produced directly in a solubilized form in the presence of MNG-3, adapted from Fogeron et al. (2015b). from publication: Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology | Cell-free protein synthesis (CFPS) systems are gaining more importance as universal tools for basic research, applied sciences, and product development with new technologies emerging for their application. Huge progress was made in the field of synthetic biology using CFPS to | Structural Biology, Wheat and Protein Expression | ResearchGate, the professional network for scientists.

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